Transcription Factor

Accessions: EOMES_DBD (HumanTF 1.0), EOMES_TF2 (HumanTF2 1.0), EOMES (HT-SELEX2 May2017)
Names: EOMES, EOMES_HUMAN, Eomesodermin homolog, T-box brain protein 2, T-brain-2, TBR-2, ENSG00000163508
Organisms: Homo sapiens
Libraries: HumanTF 1.0 1, HumanTF2 1.0 2, HT-SELEX2 May2017 3
1 Jolma A, Yan J, Whitington T, Toivonen J, Nitta KR, Rastas P, Morgunova E, Enge M, Taipale M, Wei G, Palin K, Vaquerizas JM, Vincentelli R, Luscombe NM, Hughes TR, Lemaire P, Ukkonen E, Kivioja T, Taipale J. DNA-Binding Specificities of Human Transcription Factors. Cell. 2013 Jan 17;152(1-2):327-39. [Pubmed]
2 Jolma A, Yin Y, Nitta KR, Dave K, Popov A, Taipale M, Enge M, Kivioja T, Morgunova E, Taipale J. DNA-dependent formation of transcription factor pairs alters their binding specificity. Nature 527:384-8 (2015). [Pubmed]
3 Yin Y, Morgunova E, Jolma A, Kaasinen E, Sahu B, Khund-Sayeed S, Das PK, Kivioja T, Dave K, Zhong F, Nitta KR, Taipale M, Popov A, Ginno PA, Domcke S, Yan J, Schübeler D, Vinson C, Taipale J. Impact of cytosine methylation on DNA binding specificities of human transcription factors. Science : (2017). [Pubmed]
Uniprot: O95936
Notes: Ensembl ID: ENSG00000163508; DNA-binding domain sequence; TF family: T-box; Clone source: Gene synthesis, Ensembl ID: ENSG00000163508; Construct type: TF2(3xFLAG); TF family: Tbox; Clone source: Jolma et al. 2013, TF family: T_box experiment: HT-SELEX Hamming distance: 1 cycle: 4, TF family: T_box experiment: Methyl-HT-SELEX Hamming distance: 1 cycle: 4
Length: 224
Pfam Domains: 20-208 T-box
Sequence:
(in bold interface residues)
1 GSCGGLGGLGVPSSGFRAHVYLCNRPLWLKFHRHQTEMIITKQGRRMFPFLSFNINGLNP 60
61 TAHYNVFVEVVLADPNHWRFQGGKWVTCGKADNNMQGNKMYVHPESPNTGSHWMRQEISF 120
121 GKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDLNEPSKTQTFTFSETQF 180
181 IAVTAYQNTDITQLKIDHNPFAKGFRDNYDSSHQIVPGGRYGVQ
Interface Residues: 45, 184, 185, 201, 204, 205
3D-footprint Homologues: 4a04_B, 1h6f_B, 5flv_I, 1xbr_B, 6f59_B, 2x6v_A
Binding Motifs: EOMES_DBD_1 aAGGTGTGAaawh
EOMES_DBD_2 TmACACctwskragGTGTGA
ALX4_EOMES skyGctAAytwwwktTvrCACmt
E2F1_EOMES_1 vrGTGtGaAwgGCGCsywkCac
E2F1_EOMES_2 rsGYGtsrwGGCGsywTgdCrbgg
E2F3_EOMES argYGykAtGGCGCstTmrCrcyt
ELK1_EOMES_1 rrGyGygrrrryrgkrtsrCrCCGGAAgy
ELK1_EOMES_2 rcCGGAAGTrgGyGygA
ELK1_EOMES_2_3 tmrCACCGGAAgy
ERF_EOMES_1 rgGTGtgrvvdhrdwwtvdCACmGGAArb
ERF_EOMES_2 waTmrCACMGGAwry
ETV2_EOMES_1 agGYGtkamvtksmrttsrCrcMGGAwryg
ETV2_EOMES_2 TcrCRCMGGAwry
ETV2_EOMES_2_3 aCCGGAwryrmmwhyymACACctw
ETV5_EOMES_1 rrGTGtkrdbskgrgkTsrCrsmGGAwgy
ETV5_EOMES_2 ycrCrcCGGAwg
GCM2_EOMES rTGCGGGhagGTGtga
HOXA3_EOMES TAAyGagGTGtka
HOXB13_EOMES_1 aggTGtswmATwAAa
HOXB13_EOMES_2 cymrTwAawtmaCAccy
HOXB2_EOMES_1 rgGYGyTAATkg
HOXB2_EOMES_2 trAtkrgGTGyka
HOXC10_EOMES TmaCACcTCrTaAa
HOXD12_EOMES_1 agGyGyGAmrmwamatTmACACCTCrTaAa
HOXD12_EOMES_2 tTwAYGAkswwCmTCGTwAA
HOXD12_EOMES_2_3 TmrCACcTCrTwAA
MEIS1_EOMES asgTGwkGaCAk
MYBL1_EOMES_1 mcsyGacyAACsghmyycaCrCcy
MYBL1_EOMES_2 rsGTGtshAACgg
PITX1_EOMES_1 GGATTArcrgGTGtgr
PITX1_EOMES_2 rgGTGTkAarkrGGATTA
POU2F1_EOMES_1 wTATkCAgyGtka
POU2F1_EOMES_2 rgTGtwrgaATATkcm
POU2F1_EOMES_2_3 akGTGtwkGmATATkymyrCrswg
POU2F1_EOMES_2_3_4 gawTATkCAGyGtkA
POU2F1_EOMES_2_3_4_5 gmaTATKCrGyGykAwwtmrryttmrCrCb
TEAD4_EOMES_1 ggyGybAMATwcytwtcrCrCy
TEAD4_EOMES_2 rGaATGyGtkA-
TEAD4_EOMES_2_3 wkGyGykAmATtcy
TEAD4_EOMES_2_3_4 aggyGysACATTCCd
TEAD4_EOMES_2_3_4_5 GGwATGyragGTGygr
TEAD4_EOMES_2_3_4_5_6 rgGTGtkrssrkwGGwATGyg
EOMES_2 rAGGTGTGAa
EOMES_methyl_1 dAgGTGTgAw
Publications: Jolma A, Yan J, Whitington T, Toivonen J, Nitta KR, Rastas P, Morgunova E, Enge M, Taipale M, Wei G, Palin K, Vaquerizas JM, Vincentelli R, Luscombe NM, Hughes TR, Lemaire P, Ukkonen E, Kivioja T, Taipale J. DNA-Binding Specificities of Human Transcription Factors. Cell. 2013 Jan 17;152(1-2):327-39. [Pubmed]

Jolma A, Yin Y, Nitta KR, Dave K, Popov A, Taipale M, Enge M, Kivioja T, Morgunova E, Taipale J. DNA-dependent formation of transcription factor pairs alters their binding specificity. Nature 527:384-8 (2015). [Pubmed]
Related annotations: PaperBLAST

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