Transcription Factor

Accessions: 1lb2_A (3D-footprint 20231221), 3iyd_H (3D-footprint 20231221)
Names: cAMP receptor protein, cAMP regulatory protein, cAMP-activated global transcriptional regulator CRP, CAP, Catabolite activator protein, CATABOLITE GENE ACTIVATOR PROTEIN, CRP_ECOLI, Catabolite gene activator
Organisms: Escherichia coli, strain K12
Libraries: 3D-footprint 20231221 1
1 Contreras-Moreira B. 3D-footprint: a database for the structural analysis of protein-DNA complexes. Nucleic acids research 38:D91-7 (2010). [Pubmed]
Uniprot: P0ACJ8
Length: 201
Pfam Domains: 14-102 Cyclic nucleotide-binding domain
133-197 Crp-like helix-turn-helix domain
157-188 Bacterial regulatory proteins, crp family
Sequence:
(in bold interface residues)
1 PTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGD 60
61 FIGELGLFEEGQERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTS 120
121 EKVGNLAFLDVTGRIAQTLLNLAKQPDAMTHPDGMQIKITRQEIGQIVGCSRETVGRILK 180
181 MLEDQNLISAHGKTIVVYGTR
Interface Residues: 18, 72, 157, 162, 171, 172, 173, 174, 176, 177
3D-footprint Homologues: 3iyd_H, 4a12_B, 2xro_E, 3mzh_A, 5i2d_R, 3q5f_A, 7pza_B, 3e6c_C, 4i2o_B
Binding Motifs: 1lb2_ABE TwnnnnnnnnnntGtGA
3iyd_H AnnnnTGTGaT
Binding Sites: 1lb2_J
1lb2_K
3iyd_I
3iyd_J
Publications: Benoff B., Yang H., Lawson CL., Parkinson G., Liu J., Blatter E., Ebright YW., Berman HM., Ebright RH. Structural basis of transcription activation: the CAP-alpha CTD-DNA complex. Science. 297(5586):1562-6 (2002). [Pubmed]

Hudson B.P, Quispe J, Lara-González S, Kim Y, Berman H.M, Arnold E, Ebright R.H, Lawson C.L. Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Proceedings of the National Academy of Sciences of the United States of America 106:19830-5 (2009). [Pubmed]
Related annotations: PaperBLAST

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