Transcription Factor

						

			
Accessions: 1o3q_A (3D-footprint 20231221), 1o3r_A (3D-footprint 20231221), 1zrc_B (3D-footprint 20231221), 1zrd_B (3D-footprint 20231221), 1zre_B (3D-footprint 20231221), 2cgp_A (3D-footprint 20231221)

			
Names: cAMP receptor protein, cAMP regulatory protein, cAMP-activated global transcriptional regulator CRP, CAP, Catabolite activator protein, CATABOLITE GENE ACTIVATOR PROTEIN, CRP_ECOLI, Catabolite gene activator
Organisms: Escherichia coli, strain K12
Libraries: 3D-footprint 20231221 1
1 Contreras-Moreira B. 3D-footprint: a database for the structural analysis of protein-DNA complexes. Nucleic acids research 38:D91-7 (2010). [Pubmed]
Uniprot: P0ACJ8
Length: 200
Pfam Domains:    15-103 Cyclic nucleotide-binding domain
  134-198 Crp-like helix-turn-helix domain
  158-189 Bacterial regulatory proteins, crp family
Sequence: 
(in bold interface residues)		   1 DPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQG   60
  61 DFIGELGLFEEGQERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVT  120
 121 SEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTHPDGMQIKITRQEIGQIVGCSRETVGRIL  180
 181 KMLEDQNLISAHGKTIVVYG
Interface Residues: 19, 73, 158, 163, 172, 173, 174, 175, 177, 178
3D-footprint Homologues: 3iyd_H, 4a12_B, 2xro_E, 3mzh_A, 7pza_B, 3e6c_C, 4i2o_B, 5i2d_R
Binding Motifs: 1zrd_B TgaGA
1o3q_A TGTGA
1o3r_A AAntGcGA
1zrc_B TGTGA
1zre_B TCcCA
2cgp_A tGTGAc
Binding Sites: 1o3q_B
1o3q_C
1o3r_B
1o3r_C
1zrc_Y
1zrc_Z
1zrd_Y
1zrd_Z
1zre_Y
1zre_Z
2cgp_B
2cgp_C
			
Publications: Chen S, Vojtechovsky J, Parkinson G.N, Ebright R.H, Berman H.M. Indirect readout of DNA sequence at the primary-kink site in the CAP-DNA complex: DNA binding specificity based on energetics of DNA kinking. Journal of molecular biology 314:63-74 (2001). [Pubmed]


Napoli A.A, Lawson C.L, Ebright R.H, Berman H.M. Indirect readout of DNA sequence at the primary-kink site in the CAP-DNA complex: recognition of pyrimidine-purine and purine-purine steps. Journal of molecular biology 357:173-83 (2006). [Pubmed]


Passner JM., Steitz TA. The structure of a CAP-DNA complex having two cAMP molecules bound to each monomer. Proc Natl Acad Sci U S A. 94(7):2843-7 (1997). [Pubmed]
Related annotations: PaperBLAST

	
			


			

				

				
				
				
				

			

			

		

	
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