Transcription Factor

						

			
Accessions: 1o3q_A (3D-footprint 20231221), 1o3r_A (3D-footprint 20231221), 1zrc_B (3D-footprint 20231221), 1zrd_B (3D-footprint 20231221), 1zre_B (3D-footprint 20231221), 2cgp_A (3D-footprint 20231221)

			
Names: cAMP receptor protein, cAMP regulatory protein, cAMP-activated global transcriptional regulator CRP, CAP, Catabolite activator protein, CATABOLITE GENE ACTIVATOR PROTEIN, CRP_ECOLI, Catabolite gene activator
Organisms: Escherichia coli, strain K12
Libraries: 3D-footprint 20231221 1
1 Contreras-Moreira B. 3D-footprint: a database for the structural analysis of protein-DNA complexes. Nucleic acids research 38:D91-7 (2010). [Pubmed]
Uniprot: P0ACJ8
Length: 200
Pfam Domains:    15-103 Cyclic nucleotide-binding domain
  134-198 Crp-like helix-turn-helix domain
  158-189 Bacterial regulatory proteins, crp family
Sequence: 
(in bold interface residues)		   1 DPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQG   60
  61 DFIGELGLFEEGQERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVT  120
 121 SEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTHPDGMQIKITRQEIGQIVGCSRETVGRIL  180
 181 KMLEDQNLISAHGKTIVVYG
Interface Residues: 19, 73, 158, 163, 172, 173, 174, 175, 177, 178
3D-footprint Homologues: 3iyd_H, 4a12_B, 2xro_E, 3e6c_C, 4i2o_B, 5i2d_R, 3mzh_A, 7pza_B
Binding Motifs: 1zrd_B TgaGA
1o3q_A TGTGA
1o3r_A AAntGcGA
1zrc_B TGTGA
1zre_B TCcCA
2cgp_A tGTGAc
Binding Sites: 1o3q_B
1o3q_C
1o3r_B
1o3r_C
1zrc_Y
1zrc_Z
1zrd_Y
1zrd_Z
1zre_Y
1zre_Z
2cgp_B
2cgp_C
			
Publications: Chen S, Vojtechovsky J, Parkinson G.N, Ebright R.H, Berman H.M. Indirect readout of DNA sequence at the primary-kink site in the CAP-DNA complex: DNA binding specificity based on energetics of DNA kinking. Journal of molecular biology 314:63-74 (2001). [Pubmed]


Napoli A.A, Lawson C.L, Ebright R.H, Berman H.M. Indirect readout of DNA sequence at the primary-kink site in the CAP-DNA complex: recognition of pyrimidine-purine and purine-purine steps. Journal of molecular biology 357:173-83 (2006). [Pubmed]


Passner JM., Steitz TA. The structure of a CAP-DNA complex having two cAMP molecules bound to each monomer. Proc Natl Acad Sci U S A. 94(7):2843-7 (1997). [Pubmed]
Related annotations: PaperBLAST

	
			


			

				

				
				
				
				

			

			

		

	
Disclaimer and license


These data are available AS IS and at your own risk. The EEAD/CSIC do not give any representation or warranty nor assume any liability or responsibility for the data nor the results posted (whether as to their accuracy, completeness, quality or otherwise). Access to these data is available free of charge for ordinary use in the course of research. Downloaded data have CC-BY-NC-SA license. FootprintDB is also available at RSAT::Plants, part of the INB/ELIXIR-ES resources portfolio.