Transcription Factor
| Accessions: | ECK120008667 (RegulonDB 7.5) |
| Names: | NanR, NanR DNA-binding transcriptional dual regulator |
| Organisms: | ECK12 |
| Libraries: | RegulonDB 7.5 1 1 Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muniz-Rascado L, Garcia-Sotelo JS, Weiss V, Solano-Lira H, Martinez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernandez S, Alquicira-Hernandez K, Lopez-Fuentes A, Porron-Sotelo L, Huerta AM, Bonavides-Martinez C, Balderas-Martinez YI, Pannier L, Olvera M, Labastida A, Jimenez-Jacinto V, Vega-Alvarado L, Del Moral-Chavez V, Hernandez-Alvarez A, Morett E, Collado-Vides J. RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more. Nucleic Acids Res. 2013 Jan 1;41(D1):D203-D213. [Pubmed] |
| Notes: | The genes regulated by NanR, N-acetyl-neuraminic acid regulator, are involved in N-acetyl-neuraminic acid (or sialic acid) transport and metabolism Kalivoda KA,2003and in off-to-on switching of type 1 fimbriation; NanR is inactivated by N-acetyl-neuraminic acid Sohanpal BK,2004NanR is a member of the FadR/GntR family; Members of this family have two domains, an N-terminal domain with a helix-turn-helix DNA-binding motif and a C-terminal domain with dimerization and effector-binding motifs; Three-dimensional models of the N terminus of FadR and NanR show topological similarity and a ~26% sequence identity between them Kalivoda KA,2003NanR regulates transcription when it binds to a region of ~30 bp that contains three conserved motifs in tandem; However, this protein can be displaced from this region by N-acetyl-neuraminic acid; Although it has been shown that NanR can form homodimers in solution, its DNA-binding stoichiometry is unknown; When this protein is repressing transcription, it overlaps the whole promoter region Kalivoda KA,2003; Sohanpal BK,2004The region of 30 bp that NanR binds to is close to a Dam methylation site (GATC) Chu D,2008; Sohanpal BK,2004that appears to be necessary for induction of some genes of the NanR regulon Oshima T,2002 Methylation in these sites is prevented by NanR binding Chu D,2008; Sohanpal BK,2004; Transcription related; DNA binding; sequence-specific DNA binding transcription factor activity; intracellular; regulation of transcription, DNA-dependent; protein-DNA complex; transcription, DNA-dependent; operon; repressor |
| Length: | 264 |
| Pfam Domains: | 32-95 Bacterial regulatory proteins, gntR family 125-246 FCD domain |
| Sequence: (in bold interface residues) | 1 MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMIRRREFGEGEQLPSERE 60 61 LMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADTIIGELSGMAKDFLSHPGG 120 121 IAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQSLDNNAAFIRSDVDFHRVLA 180 181 EIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVSYQQHIAIVDAIRRHDPDEADRA 240 241 LQSHLNSVSATWHAFGQTTNKKK* |
| Interface Residues: | 57, 58, 59, 62, 68, 69, 70, 71, 72, 73, 74, 89, 91, 92 |
| 3D-footprint Homologues: | 6wg7_G, 4h0e_A, 4p9u_F, 4wwc_B, 4u0y_B, 1h9t_B, 7bhy_A, 5vvr_M |
| Binding Motifs: | NanR AmAGG |
| Binding Sites: | ECK120012561 ECK120012563 ECK120012565 ECK120012569 ECK120012571 ECK120012573 |
| Publications: | Chu D., Roobol J., Blomfield IC. A theoretical interpretation of the transient sialic acid toxicity of a nanR mutant of Escherichia coli. J Mol Biol. 375(3):875-89 (2008). [Pubmed] Oshima T., Wada C., Kawagoe Y., Ara T., Maeda M., Masuda Y., Hiraga S., Mori H. Genome-wide analysis of deoxyadenosine methyltransferase-mediated control of gene expression in Escherichia coli. Mol Microbiol. 45(3):673-95 (2002). [Pubmed] Sohanpal BK., El-Labany S., Lahooti M., Plumbridge JA., Blomfield IC. Integrated regulatory responses of fimB to N-acetylneuraminic (sialic) acid and GlcNAc in Escherichia coli K-12. Proc Natl Acad Sci U S A. 101(46):16322-7 (2004). [Pubmed] Kalivoda KA., Steenbergen SM., Vimr ER., Plumbridge J. Regulation of sialic acid catabolism by the DNA binding protein NanR in Escherichia coli. J Bacteriol. 185(16):4806-15 (2003). [Pubmed] |
| Related annotations: | PaperBLAST |
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