Transcription Factor

						

			
Accessions: 1cgp_A (3D-footprint 20231221), 1cgp_B (3D-footprint 20231221), 1j59_B (3D-footprint 20231221), 1o3t_B (3D-footprint 20231221), 1run_B (3D-footprint 20231221)

			
Names: cAMP receptor protein, cAMP regulatory protein, cAMP-activated global transcriptional regulator CRP, Catabolite activator protein, CATABOLITE GENE ACTIVATOR PROTEIN (CAP), CRP_ECOLI, CAP, Catabolite gene activator, CATABOLITE GENE ACTIVATOR PROTEIN
Organisms: Escherichia coli, strain K12
Libraries: 3D-footprint 20231221 1
1 Contreras-Moreira B. 3D-footprint: a database for the structural analysis of protein-DNA complexes. Nucleic acids research 38:D91-7 (2010). [Pubmed]
Uniprot: P0ACJ8
Length: 197
Pfam Domains:    14-102 Cyclic nucleotide-binding domain
  133-197 Crp-like helix-turn-helix domain
  157-188 Bacterial regulatory proteins, crp family
Sequence: 
(in bold interface residues)		   1 PTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGD   60
  61 FIGELGLFEEGQERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTS  120
 121 EKVGNLAFLDVTGRIAQTLLNLAKQPDAMTHPDGMQIKITRQEIGQIVGCSRETVGRILK  180
 181 MLEDQNLISAHGKTIVV
Interface Residues: 18, 72, 157, 162, 171, 172, 173, 174, 176, 177
3D-footprint Homologues: 3iyd_H, 4a12_B, 2xro_E, 3q5f_A, 7pza_B, 3e6c_C, 4i2o_B, 3mzh_A, 5i2d_R
Binding Motifs: 1o3t_B tCgCA
1cgp_AB TGTGACnnnnTTCACA
1cgp_B TgTgAc
1j59_B GTCACA
1run_B ATcaCA
Binding Sites: 1cgp_E
1j59_D
1j59_E
1o3t_E
1o3t_F
1run_D
1run_E
1cgp_C
1cgp_F
			
Publications: Schultz SC., Shields GC., Steitz TA. Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees. Science. 253(5023):1001-7 (1991). [Pubmed]


Parkinson G., Wilson C., Gunasekera A., Ebright YW., Ebright RE., Berman HM. Structure of the CAP-DNA complex at 2.5 angstroms resolution: a complete picture of the protein-DNA interface. J Mol Biol. 260(3):395-408 (1996). [Pubmed]


Chen S, Vojtechovsky J, Parkinson G.N, Ebright R.H, Berman H.M. Indirect readout of DNA sequence at the primary-kink site in the CAP-DNA complex: DNA binding specificity based on energetics of DNA kinking. Journal of molecular biology 314:63-74 (2001). [Pubmed]


Parkinson G, Gunasekera A, Vojtechovsky J, Zhang X, Kunkel T.A, Berman H, Ebright R.H. Aromatic hydrogen bond in sequence-specific protein DNA recognition. Nature structural biology 3:837-41 (1996). [Pubmed]
Related annotations: PaperBLAST

	
			


			

				

				
				
				
				

			

			

		

	
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