Transcription Factor

						

			
Accessions: 6ee8_F (3D-footprint 20231221), 6m7j_F (3D-footprint 20231221), 6vvx_F (3D-footprint 20231221), 6vvz_F (3D-footprint 20231221)

			
Names: RNA polymerase sigma factor SigA, SIGA_MYCTU, Sigma-A
Organisms: Mycobacterium tuberculosis, strain ATCC 25618 / H37Rv
Libraries: 3D-footprint 20231221 1
1 Contreras-Moreira B. 3D-footprint: a database for the structural analysis of protein-DNA complexes. Nucleic acids research 38:D91-7 (2010). [Pubmed]
Length: 319
Pfam Domains:     17-51 Sigma-70 factor, region 1.2
   87-157 Sigma-70 region 2 
  166-242 Sigma-70 region 3
  255-308 Sigma-70, region 4
Sequence: 
(in bold interface residues)		   1 SEALRQARKDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYATQLMTELSE   60
  61 RGEKLPAAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGL  120
 121 IRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQ  180
 181 DLGREPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAV  240
 241 DAVSFTLLQDQLQSVLDTLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIES  300
 301 KTMSKLRHPSRSQVLRDYL
Interface Residues: 281, 282, 291, 292, 293, 294, 296, 297
3D-footprint Homologues: 3n97_A, 6ido_B, 8cyf_B
Binding Motifs: 6ee8_F CTTGACTnnnnnnnnnnnnnTnnnTTA
6m7j_F AGCanAnnnnnnnnnnnntGTCAAG
6vvx_CDFM TGCGTnTAAnAnAnnnnnnnnnnnnnAGTCAAGA
6vvz_CDFM TCTTGACTnnnnnnnnnnnnnTnTnTTAGACCA
6vvz_F TCTTGACTnnnnnnnnnnnnnTnTnTTAGAC
Binding Sites: 6m7j_O
6m7j_P
6ee8_P
6ee8_O
6vvz_O
6vvz_P
			
Publications: Boyaci H, Chen J, Jansen R, Darst SA, Campbell EA. Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding. Nature 565:382-385 (2019). [Pubmed]


Lilic M, Chen J, Boyaci H, Braffman N, Hubin EA, Herrmann J, Müller R, Mooney R, Landick R, Darst SA, Campbell EA. The antibiotic sorangicin A inhibits promoter DNA unwinding in a Mycobacterium tuberculosis rifampicin-resistant RNA polymerase. Proc Natl Acad Sci U S A 117:30423-30432 (2020). [Pubmed]
Related annotations: PaperBLAST

	
			


			

				

				
				
				
				

			

			

		

	
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